Detection of Brucella spp. in dogs at Pantanal wetlands
نویسندگان
چکیده
منابع مشابه
Comparison of Dot-ELISA with microbial culture for detection of Brucella spp. in clinical specimens
Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. The specimens were also examined after centrifugation by ...
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Background: Classical bacteriological detection of Brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. However, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. Methods: Panel of some reference strains belonging to ...
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Ticks harbor many pathogenic, as well as endosymbiotic and non-pathogenic agents. They are host of a variety of as yet unidentified microbes that continue to be described. In the present study, a Brucella-like bacterium was detected in a Boophilus tick by PCR amplification of a partial fragment of 16S rRNA locus followed sequencing. Our results show that the members of the genus Boophilus may a...
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Here we present a review of most of the currently used polymerase chain reaction (PCR)-based methods for identification of Brucella bacteria in biological samples. We focused in particular on methods using single-pair primers, multiplex primers, real-time PCRs, PCRs for marine Brucella, and PCRs for molecular biotyping. These methods are becoming very important tools for the identification of B...
متن کاملTicks on humans in the Pantanal wetlands, Brazil.
Information on ticks biting humans in Brazil is very restricted. In fact, many times when human tick-borne diseases are diagnosed, the involved vector tick is not identified, although this may be clinically helpful. Pantanal is one of the world's largest floodplains, has an exuberant wildlife, and is place of extensive cattle ranching, ecotourism, and fishing. We herein report tick species foun...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2018
ISSN: 1517-8382,1678-4405
DOI: 10.1007/s42770-018-0006-5